Claire Ortmann
I will provide an overview of the Correlative Light and Electron Microscopy (CLEM) workflow established in our institute and the different variations possible in our facility. This workflow encompasses the screening of vitrified whole cells on our cryo-confocal microscope, thinning of the cells in a FIB-SEM to a lamella of less than 300 nm, imaging of thin lamella in a cryo-fluorescence microscope and finally cryo-electron tomography on the same lamella in a correlated fashion. I will also give a brief overview of different correlation softwares available for this workflow.